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Kentucky Bioassays and Biomonitoring: What you need to know

Governing Law and Regulations

Whole effluent toxicity (WET) testing: 401 Kentucky Administrative Regulations (KAR) 5:030

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Permit conditions: 401 KAR 5:065

Laboratory certification:

Public water systems: 401 KAR 8:040

Petroleum tank laboratories: 401 KAR 42:340

Wastewater: 401 KAR 5:320

Regulatory Agencies

Kentucky Energy and Environment Cabinet Department for Environmental Protection (DEP) Division of Water

Kentucky Energy and Environment Cabinet DEP Division of Environmental Services

U.S. Environmental Protection Agency (EPA) Region 4

See ADDRESSES & CONTACTS for addresses and telephone numbers.

See national section for basic information and federal regulations.

Comparison: State vs. Federal

Rules. Kentucky generally follows the federal guidelines in requiring Kentucky Pollutant Discharge Elimination System (KPDES) applicants to include biological monitoring data in requiring bioassays and biomonitoring to demonstrate compliance with water quality standards.

All KPDES permit applicants must provide an identification of any whole effluent toxicity (WET) tests conducted during the 4 1/2 years before the date of the application on any of the applicant's discharges or on any receiving water near the discharge. Applicants must submit the results of valid WET tests to the state for acute or chronic toxicity for samples taken from each outfall through which effluent is discharged to surface waters.

Kentucky has also established requirements for certifying laboratories involved in testing public water supplies and wastewater in the state. This includes specific standards for laboratories that work on petroleum storage tank projects.

Administration and enforcement.DEP’s Division of Water administers and enforces the state’s water ...


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State Requirements

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Kentucky Bioassays and Biomonitoring Resources

Bioassays and Biomonitoring Products

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