Colorado Bioassays and Biomonitoring regulations & environmental compliance analysis

Colorado Bioassays and Biomonitoring: What you need to know

Governing Law and Regulations

Colorado Pollutant Discharge System (CPDS) permits: 5 Code of Colorado Regulations (CCR) 1002-61.2

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Whole effluent toxicity (WET) testing: 5 CCR 1002-61.26

Permits: 5 CCR 1002-62.8(2)(b)

Laboratory certification: 5 CCR 1005-1

Regulatory Agencies

Colorado Department of Public Health and Environment (CDPHE) Office of Environment Water Quality Control Division

CDPHE Office of Environment Laboratory Services Division

U.S. Environmental Protection Agency (EPA) Region 8

See ADDRESSES & CONTACTS for addresses and telephone numbers.

See national section for basic information and federal regulations.

Comparison: State vs. Federal

Rules. Colorado requires site-specific water standards when justified by the results of a bioassay or comparable scientific study. State guidelines for developing site-specific aquatic life criteria are comparable to methods established by the U.S. Environmental Protection Agency (EPA).

Like the federal rules, whole effluent toxicity (WET) tests may be incorporated into a facility's Colorado Pollutant Discharge System (CPDS) permit (5 CCR 1002-61.2). The CPDS permit is the state's equivalent to the National Pollutant Discharge Elimination System permit. See the state section NPDES PERMIT for more information.

The state defines "WET" as a biological activity effect by which effluents exhibit antagonism to the aquatic organisms used in biomonitoring tests in the form of acute or chronic toxicity. WET may be caused by a variety of specific compounds or by synergistic interaction among compounds.

Administration and enforcement. CDPHE's Water Quality Control Division is responsible for establishing monitoring requirements as part of the permit and for enforcing compliance with ...


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