Indiana Bioassays and Biomonitoring regulations & environmental compliance analysis

Indiana Bioassays and Biomonitoring: What you need to know

Governing Law and Regulations


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Waters Not Within the Great Lakes System: 327 IAC 2-1-8 to 2-1-8.9 and 327 IAC 5-2-11.1

Waters Within the Great Lakes System: 327 IAC 2-1.5-10 to 2-1.5-15 and 327 IAC 5-2-11.4

Publicly owned treatment works (POTW) permits: 327 IAC 5-2-3

Regulatory Agencies

Indiana Department of Environmental Management (IDEM) Office of Water Quality

Indiana State Department of Health (ISDH)

U.S. Environmental Protection Agency (EPA) Region 5

See ADDRESSES & CONTACTS for addresses and telephone numbers.

See national section for basic information and federal regulations.

Comparison: State vs. Federal

Rules. Indiana generally follows the federal rules for bioassays and biomonitoring and may require testing whenever there is evidence of unacceptable levels of effluent toxicity. See the national section BIOASSAYS/BIOMONITORING for more information.

Administration and enforcement. IDEM's Office of Water Quality is responsible for the administration and enforcement of all water monitoring programs. ISDH Laboratories are responsible for certifying laboratories under the Safe Drinking Water Act (SDWA), including conducting on-site evaluations and evaluating proficiency test samples. ISDH also maintains lists of certified labs.

State Requirements


The state has established separate regulations for state waters within the Great Lakes system and those that are not.

Waters Not Within the Great Lakes System

327 IAC 2-1-8 to 2-1-8.9 and 327 IAC 5-2-11.1

The analytical procedures used as methods of analysis to determine the chemical, bacteriological, biological, and radiological quality of state waters not located within the Great Lakes system must be performed in accordance with 40 CFR 136 or ...

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