Wisconsin Bioassays and Biomonitoring regulations & environmental compliance analysis

Wisconsin Bioassays and Biomonitoring: What you need to know

Governing Law and Regulations

Whole effluent toxicity (WET) testing: Wisconsin Administrative Code, Department of Natural Resources (NR) 106.08 and NR 106.09

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Laboratory certification: NR 149.01 to 149.49

Applications: NR 149.14

Laboratory evaluations: NR 149.29 to 149.34

Standard operating procedures: NR 149.40

Regulatory Agencies

Wisconsin Department of Natural Resources (DNR) Division of Water

DNR Laboratory Certification and Registration Program

U.S. Environmental Protection Agency (EPA) Region 5

See ADDRESSES & CONTACTS for addresses and telephone numbers.

See national section for basic information and federal regulations.

Comparison: State vs. Federal

Rules. Wisconsin regulations allow DNR to establish whole effluent toxicity (WET) limitations and to require bioassays and biomonitoring when necessary to meet water quality standards. The state generally follows the federal regulations for WET testing and also has some additional requirements for laboratory certification. See the national section BIOASSAYS/BIOMONITORING for additional information about the federal requirements.

Administration and enforcement. DNR's Division of Water administers and enforces the state's water quality management program, including for WET testing. DNR's Laboratory Certification and Registration Program oversees the enforcement of the laboratory certification regulations.

State Requirements


Under the Wisconsin Pollutant Discharge Elimination System (WPDES) permit program, two types of effluent limits are established: categorical effluent limits and water quality-based effluent limits.

Categorical effluent limits are based on technology standards, such as the kind of technology that can achieve pollution control. ...

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Wisconsin Bioassays and Biomonitoring Resources

Type Title
Checklists Quality Assurance Manual Checklist
Forms Whole Effluent Toxicity (WET) Test Report
See all Bioassays and Biomonitoring Resources